Composite
Part:BBa_K3002200:Design
Designed by: Dorothée Klein Group: iGEM19_TU_Kaiserslautern (2019-10-21)
L2 spectinomycin resistance + Mut-PETase + MHETase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5370
Illegal PstI site found at 3558
Illegal PstI site found at 4531
Illegal PstI site found at 6785
Illegal PstI site found at 7109
Illegal PstI site found at 7452
Illegal PstI site found at 8262 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5370
Illegal NheI site found at 2680
Illegal NheI site found at 5649
Illegal PstI site found at 3558
Illegal PstI site found at 4531
Illegal PstI site found at 6785
Illegal PstI site found at 7109
Illegal PstI site found at 7452
Illegal PstI site found at 8262
Illegal NotI site found at 7120 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5370
Illegal BglII site found at 8030 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5370
Illegal PstI site found at 3558
Illegal PstI site found at 4531
Illegal PstI site found at 6785
Illegal PstI site found at 7109
Illegal PstI site found at 7452
Illegal PstI site found at 8262 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5370
Illegal PstI site found at 3558
Illegal PstI site found at 4531
Illegal PstI site found at 6785
Illegal PstI site found at 7109
Illegal PstI site found at 7452
Illegal PstI site found at 8262
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3293
Illegal NgoMIV site found at 3320
Illegal NgoMIV site found at 4971
Illegal NgoMIV site found at 6718 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part is a combination of the parts BBa_K3002102 (spectinomycin resistance), BBa_K3002105 (Mutant PETase) and BBa_K3002112 (wildtype MHETase).