Composite

Part:BBa_K3002200:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 spectinomycin resistance + Mut-PETase + MHETase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5370
    Illegal PstI site found at 3558
    Illegal PstI site found at 4531
    Illegal PstI site found at 6785
    Illegal PstI site found at 7109
    Illegal PstI site found at 7452
    Illegal PstI site found at 8262
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5370
    Illegal NheI site found at 2680
    Illegal NheI site found at 5649
    Illegal PstI site found at 3558
    Illegal PstI site found at 4531
    Illegal PstI site found at 6785
    Illegal PstI site found at 7109
    Illegal PstI site found at 7452
    Illegal PstI site found at 8262
    Illegal NotI site found at 7120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5370
    Illegal BglII site found at 8030
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5370
    Illegal PstI site found at 3558
    Illegal PstI site found at 4531
    Illegal PstI site found at 6785
    Illegal PstI site found at 7109
    Illegal PstI site found at 7452
    Illegal PstI site found at 8262
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5370
    Illegal PstI site found at 3558
    Illegal PstI site found at 4531
    Illegal PstI site found at 6785
    Illegal PstI site found at 7109
    Illegal PstI site found at 7452
    Illegal PstI site found at 8262
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3293
    Illegal NgoMIV site found at 3320
    Illegal NgoMIV site found at 4971
    Illegal NgoMIV site found at 6718
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002102 (spectinomycin resistance), BBa_K3002105 (Mutant PETase) and BBa_K3002112 (wildtype MHETase).

References